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In distinction to random primers, oligo (dT) primers are usually not ideal for degraded samples but are exceptional for setting up cDNA libraries and proposed to be used with reverse transcriptases (RTs) as Superscript IV RT.
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Pools of pre-intended primers will help generate detailed expression profiles, Specifically in which assay sensitivity is critically significant.
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Enable The most amount website of PCR targets (amplicons) for being demonstrated when examining specificity for pre-built primers. Max targets per sequence
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In distinction to random primers, oligo (dT) primers are not ideal for degraded samples but are outstanding for constructing cDNA libraries and encouraged to be used with reverse transcriptases (RTs) as get more info Superscript IV RT.